BD Micro, GUIDE ENTEROTUBE II INTERPRETATION GUIDE, Enterotube, Each. BD Micro, GUIDE ENTEROTUBE II INTERPRETATION GUIDE, Enterotube. Remarks: Glucose – Any degree of yellow is positive. Acid end products from glucose fermentation turn the pH indicator from red (alkaline) to yellow (acid). of a Multitest System (Enterotube) for Enterotube, a multiple-test system which combines nine biochemical tests useful in the identification . (ii) Modified lysine-lactose me- dium: a .. Bergey’s manual of determinative bacteriology.

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Prepare one sheet from the coding pad for inherpretation isolate by entering patient name, specimen number, and date. However, this strain is useful as a control organism to detect negative reactions in the glucose and gas compartments. Objective The goal of this lab and next week s is to show you how to deal with samples that might come from individuals infected with.

After completing this project the students should be able to:. Identification of Gram negative bacteria should be made with the consideration of additional characteristics such as source of intterpretation, history of the patient, colonial and microscopic morphology, serology and antimicrobial susceptibility patterns.

We proposed in the Basic Microbiology. American Society for Microbiology, Washington, D. Instruct students to put on lab coats and More information. ihterpretation

If no color change, or an orange color, is observed in the glucose compartment and growth is evident by color changes in other compartments, the organism is not a member of the family Enterobacteriaceae. With the exception of indole and VP, read the reactions in a sequential fashion by comparing the colors of the media in the tube after incubation with those given in the color scheme on the cover of the coding pad and eventually with an uninoculated BBL Enterotube II which must be brought to room temperature first Figure 5.

A positive test is indicated by development of a red color in the added reagent within 10 sec.


INSTRUCTIONS FOR USE BBL Enterotube II. IA Rev.: Jan. 2007

Any sign of yellow should be interpreted as a positive reaction; orange should be considered negative. To determine the carbohydrate class of an unknown by carrying out a series of chemical reactions with the unknown and known compounds in each class More information. Metabolism sum of all chemical processes More information. A copper to brownish discoloration is read as negative.

Add circled numbers in the bracketed section and enter the sum in the space provided below the arrow Figure 6. Any reagent having a dark brown color should not be used. It consists of several steps. Once we have obtained any growth, work flow in the Microbiology.

H Biochemical characterization of Citrobacter freundii and Citrobacter diversus. Reinsert wire without sterilizing into BBL Enterotube II, using a turning motion through all 12 compartments, until the notch on the wire is aligned with the opening of the tube Figure 3.

Potter Microbiology December 4, Introduction: Davis Biochemical and serological characterization of hydrogen sulfide producing variants of Escherichia coli. The purpose of this laboratory exercise is to explore the differences between Staphylococcal species and More information.

Any degree of yellow should be interpreted as Gas production wax not lifted wax lifted a positive reaction; orange should be considered negative. The urease test is strongly positive for Proteus species and may be evident as early as 4 to 6 h after incubation; it is weakly positive light pink color after 18 to 24 h incubation for Klebsiella and Enterobacter species.

Virulence of G negatives? This test detects the formation of pyruvic acid from the deamination of phenylalanine. Amyl or Isoamyl Alcohol 75 ml p-dimethylaminobenzaldehyde 5 g Hydrochloric acid, concentrated 25 ml Dissolve p-dimethylaminobenzaldehyde the reagent should be light yellow in color in alcohol and then slowly add the acid.

When preparing and handling indole and VP reagents, observe appropriate handling instructions and Risk and Safety Phrases. Examples of oxidase-negative non-enterobacteriaceae that fail to ferment glucose under anaerobic conditions in BBL Enterotube II are glucose negative Acinetobacter species. Differentiation of the Species Staphylococcus and Streptococcus Purpose: The tubes may be inoculated up to the expiration date and incubated for the recommended incubation times.


The medium remains yellow if decarboxylation of ornithine does not occur.

Biol Lab Manual: Interpretation of Enterotube II

Urine Culture Melissa Schreiber, Presenter: The resulting combination of reactions, together with the Interpretation Guide codebookallow identification of clinically significant Enterobacteriaceae. The medium is covered with wax to provide anaerobic conditions and to allow detection of gas formation. Practice microbial collection techniques Describe colony morphology and the relationship to microbial identification.

Test preparation More information.


Madi, and Ejterotube H. Biochemical identification of bacteria Dr. This is a group activity. After completing this project the students should be able to: These chemical reactions make up the More information.

Three of these tubes will contain. GRAM negative pathogens cocci and bacilli. The hydrogen sulfide reacts with the iron salts also present in the medium to form a black precipitate of ferric sulfide usually along the line of inoculation. Identification of rare isolates should be repeated or additional testing performed to verify the identification of such organisms.

Culture media are used for recognition and identification diagnosis of microorganisms.

Bacteria are single-celled organisms that are able More information. Review Lab Report questions from previous week.

Kovacs indole reagent Indole Dropper Reagent, 50 ampoules, cat. For inoculation, incubation, and reading, proceed as described in Test Procedure.

A total of ten organisms were tested in duplicate over three days by three independent laboratories using the BBL Enterotube II. Five Biochemical characterization of Serratia liquefaciens and Serratia rubidaea.