CHROMID VRE PDF
We compared ChromID VRE medium with Enterococcosel containing vancomycin for the detection of vancomycin-resistant Enterococcus in 1, specimens. The new chromogenic agar chromID VRE (cIDVRE; bioMérieux) was compared with bile esculin agar (BD) containing 6 mg/liter vancomycin for the detection of. The performance of the recently developed Seeplex VRE detection kit was compared with chromID VRE agar and the conventional blood agar plate with.
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ChromID VRE in combination with Gram straining provided a higher chro,id than Enterococcosel, irrespective of the incubation time and enrichment. Glycopeptide-acquired resistance has emerged in Enterococcus faecalis and Enterococcus faeciumwhich are designated vancomycin-resistant Enterococcus species VRE. The resistance phenotype VanA is the most common and features high-level resistance to both vancomycin and teicoplanin.
In Europe, a high prevalence has also been observed in the United Kingdom Since then, at least five others have been reported in French hospitals 8 Early detection of VRE in fecal specimens is important for nosocomial prevention measures, epidemiologic infectious disease follow-up, and also prevention of vancomycin-resistant Staphylococcus aureus emergence 10 Several agar and broth medium formulations containing vancomycin have been developed for this purpose 135111318 However, no consensus has been established for medium base, vancomycin concentration, or method of use.
ChromID VRE is a new selective chromogenic medium developed for the detection and identification of vancomycin-resistant E. The aims of this study were to assess the performance of chromID VRE medium in a low-prevalence context and to determine the best method of use. Enterococci had brownish black to black halos on Enterococcosel with vancomycin BEAv.
Vancomycin and teicoplanin MICs were determined by E-test for resistant strains. These VRE were E. The large number of samples investigated during this study and the low prevalence of VRE provided the opportunity to explore the false positives.
The rate of VRE detection was significantly increased by the enrichment step, as previously demonstrated 711 However, fecal carriage of these VRE was transitory in patients and this increased sensitivity may not be essential for the management of most outbreaks 4.
The comparison of our results with those of other studies is difficult since in most of the latter direct plating was used without enrichment methods. The same sensitivity was found in our laboratory for VCA3 agar 5.
bioMérieux – Culture Media | product – CHROMID® VRE
Most gram-positive bacteria were isolated on BEAv after 48 h of incubation Fig. For the same response delay, reading of chromID VRE at 24 h of incubation with enrichment provided better performance than reading at 48 h of incubation after direct plating.
These conditions allowed the best compromise between sensitivity and specificity. The recommendations from this study are to incubate in chromID VRE for 24 h and perform Gram staining of typically colored strains. National Center for Biotechnology InformationU.
CHROMID® VRE – clinical diagnostics products | bioMérieux Clinical Diagnostics
Journal List J Clin Microbiol v. Published online Jun 6. Author information Article notes Copyright and License information Disclaimer. Dunant, Clermont-Ferrand, France.
This article has been cited by other articles in PMC. Open in a separate window. Evaluation of selective and enrichment media for isolation of glycopeptide-resistant enterococci from faecal specimens. Gopal Rao, and B. Comparison of agar-based media for the primary isolation of glycopeptide-resistant enterococci. Guidelines for the control of glycopeptide-resistant enterococci in hospitals. Evaluation of a new molecular system for simultaneous identification of four Enterococcus species and their glycopeptide resistance genotypes.
Neomycin blood agar as a selective medium for vancomycin resistant Enterococcus faecium. Le portage des enterocoques resistants aux glycopeptides ERV: European survey of vancomycin-resistant enterococci in at-risk hospital wards and in vitro susceptibility testing of ramoplanin against these isolates. Recommendations for preventing the spread of vancomycin resistance.
Comparison of direct plating and broth enrichment culture for the detection of intestinal colonization by glycopeptide-resistant enterococci among hospitalized patients.
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Emerging resistance among bacterial pathogens in the intensive care unit: Comparison of five selective media for identifying fecal carriage of vancomycin-resistant enterococci. Outbreak of colonization and infection with vancomycin-resistant Enterococcus faecium in a French university hospital. First nosocomial outbreak vrd vancomycin-resistant Enterococcus faecium expressing a VanD-like phenotype associated with a vanA genotype.